Arrange the steps that would be used in a laboratory to engineer a bacterium that could express the human gene coding for factor VIII. (Not all steps will be placed). Transcribed Image Text: Isolate the human gene that produces factor VIII.
Generate CDNA of the factor VIII gene using reverse transcriptase.
Insert the factor VIII CDNA into a bacterial vector near a promoter site.
Transform the vector into an E. coli bacterium.
The factor VIII protein is isolated from human tissues.
E. coli expresses factor VIII.
Answer Bank
Isolate the mRNA of the factor VIII gene.
Human DNA is introduced into the bacterial cell using a projectile gun and DNA-coated tungsten.
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