After infection, some T cells (a particular type of immune cell) may persist as memory T cells (T M cells). When they re-encounter the same pathogen, T M cells rapidly divide to produce more T cells to fight off the infection. This rapid cell division requires substantial energy. One way that this energy is provided to T M cells is via increased activity of the ETC. One standard type of experiment for analyzing oxidative metabolism is measuring the oxygen consumption rate OCR in the presence of different compounds. The lines indicate the time at which the different ETC/OxPhos inhibitors were added. Note that the inhibitors are never removed. Complete the table below. Time Period 1 2 3 4 Inhibitor added None Oligomycin (inhibits ATP Synthase) FCCP (Uncoupler) Rotenone + Antimycin (Complexes I and III) Can electrons flow? If so, from which compound to which compound? If not, what is preventing it? Can H + move through the membrane? If so, what is facilitating the crossing? If not, what is preventing it? ATP Synthesis via OxPhos? Transcribed Image Text: Oxygen consumption rate of Tm cells
400
Rotenone &
350
FCCP added
Antimycin A added
Oligomycin
added
300
250
1
3
4
200
150
100
50
20
40
60
80
100
120
Time (min)
OCR (pMole/min)
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